Road markings are an integral part of traffic signaling and they represent a set of longitudinal and transversal lines, inscriptions and symbols that shape the surface of the traffic infrastructure. Their main purpose is to ensure the safe driving for all traffic participants and to warn them of dangerous spots on the road. By using retroreflection of road markings during night and in conditions of reduced visibility, it is possible to achieve better visibility, detection on time and recognition of potentially dangerous situations. The aim of this graduate thesis is to determine whether there is a statistically significant correlation between the retroreflection of road markings and traffic accidents on the state roads of the Republic of Croatia in the period 2013-2015. In conducted analysis, the data about traffic accidents with consequences and the level of the retroreflection were taken into account, as well as the parameter of average annual daily traffic. The obtained data confirmed the initial assumption and it was concluded that by lowering the retroreflection coefficient of the road markings, the visibility is being significantly reduced and the number of traffic accidents increased.