Organization is an essential segment of every enterprise, as well as companies engaged in offering the transport of goods by train. Successful business operations depend on the organizational structure that greatly facilitates our operations due to clearly defined competencies and tasks that lead us to the ultimate goal of transporting goods. This final work tries to define and describe the organization of transporting goods by train, as well as the impact of the liberalization of the market for transporting goods on the organization of the company. Different organizational structures with possible application in railway traffic have also been described. The organization of HŽ Cargo d.o.o is presented in this work, and the activities performed by individual organizational units have also been described.